Area: Cancer Biology
Duration: Three Years
Start date: As soon as possible
Salary: $45,000/year – negotiable based on qualifications and experience
The Arnie Charbonneau Cancer Institute in the Cumming School of Medicine at the University of Calgary is accepting applications for a Postdoctoral Fellow in cancer biology.
The laboratories of Drs. Pinaki Bose and Douglas Mahoney are looking for an outstanding postdoctoral fellow for a three-year term with the possibility of extension based on funding availability. Our overall goal is to identify and characterize genes and pathways that regulate lymph node metastasis in oral cancer. We will employ a CRISPR-Cas9-based genome-wide screening approach to identify metastatic regulators, followed by functional genomic characterization of screen hits. The candidate will have access to patient-derived tissue samples and tissue microarrays containing normal, primary tumour and metastasis tissues. These will be used to validate screen hits and develop prognostic and therapeutic biomarkers. There will also be opportunities to perform in vivo screens in xenograft mouse models.
Dr. Bose is the director of translational research for the Ohlson Research Initiative (ORI). The ORI is a clinical and translational head and neck cancer research program within the Arnie Charbonneau Cancer Institute (ACCI), University of Calgary. The Bose lab is investigating mechanisms underlying head and neck carcinogenesis and progression. Dr. Mahoney is an expert in oncolytic virus therapy and directs a fully automated high-throughput screening facility within ACCI. The successful candidate will work in close collaboration with the Bose and Mahoney labs.
Although funding is available for a three-year period to support this position, the candidate will be encouraged to apply for external, peer-reviewed fellowships. The successful candidate will be enrolled in a comprehensive benefits program designed to promote a productive level of health and well-being in Postdoctoral Scholars through coverage for - health, dental, life insurance, Accidental Death & Dismemberment, and income protection for disability. All qualified candidates are encouraged to apply.
The responsibilities of this position will include:
- Develop and optimize in vitro assays for invasion and metastasis
- Develop xenograft models of lymph node metastasis
- Generate oral cancer cell lines stably-expressing Cas9 and other proteins
- Develop and execute CRISPR-Cas9-based screens
- Analyze next-generation sequencing (NGS) data to interpret the read-out of CRISPR-Cas9-based screens
- Analyze NGS data from existing databases such as the Cancer Genome Atlas (TCGA), etc.
- Prepare manuscripts, abstracts and presentations
- Applicants should have a PhD in molecular biology, cellular biology, molecular genetics, genomics or related field
- Experience with core molecular and cell biology techniques
- A detailed understanding of human molecular genetics and cancer biology
- Experience working with the CRIPSR-Cas9 system
- An excellent record of peer-reviewed publications
- Excellent organizational, written and verbal communication skills
In addition to the above, the candidate should ideally have:
- Experience with developing and performing experiments with xenograft models
- Experience performing high-throughput functional genetic screens
- Experience working with human genomic data, particularly next generation sequencing data (WGS, RNAseq)
Applications should be submitted by email to the following contact, with the subject line ‘CRISPR Screening Postdoc Position’. Please include a cover letter, your academic CV, and the names and contact information of at least three external references:
Dr. Pinaki Bose
Director, Tumour Biology and Translational Research, Ohlson Research Initiative
Arnie Charbonneau Cancer Institute
Departments of Biochemistry & Molecular Biology and Surgery
University of Calgary
Mailing Address: Room 328, HMRB
3330 Hospital Drive NW
Calgary, AB, Canada T2N 4N1
All qualified candidates are encouraged to apply; however, Canadian citizens and permanent residents will be given priority.
The application deadline is January 15, 2017.
To learn more about postdoctoral scholar opportunities at the University of Calgary and all we have to offer, view our Postdoc Careers website.
About the University of Calgary
The University of Calgary is Canada’s leading next-generation university – a living, growing and youthful institution that embraces change and opportunity with a can-do attitude. Located in the nation’s most enterprising city, the university is making tremendous progress on its Eyes High journey to become one of Canada’s top five research universities, grounded in innovative learning and teaching and fully integrated with the community it both serves and leads. Ranked as the top young university in Canada and North America, the University of Calgary inspires and supports discovery, creativity and innovation across all disciplines. For more information, visit ucalgary.ca.
We’re increasing our scholarly capacity by investing in people who want to change the world, bringing the best and brightest to Calgary to form a global intellectual hub and achieve advances that matter to everyone.
About Calgary, Alberta
Named a cultural capital of Canada and one of the best places to live in the world, Calgary is a city of leaders – in business, community, philanthropy and volunteerism. Calgarians benefit from the strongest economy in the nation and enjoy more days of sunshine per year than any other major Canadian city. Calgary is less than an hour’s drive from the Rocky Mountains and boasts the most extensive urban pathway and bikeway network in North America.
The University of Calgary believes that a respectful workplace, equal opportunity and building a diverse workforce contribute to the richness of the environment for teaching, learning and research, and provide faculty, staff, students and the public with a university that reflects the society it serves. We encourage all qualified applicants to apply, however preference will be given to Canadian citizens and permanent residents of Canada.